micrococcus luteus test results
Micrococcus luteus, the type species of the genus Micrococcus (family Micrococcaceae, order Micrococcales), is a high GC Gram-positive coccus of the phylum Actinobacteria []. Rapid identification: 5. Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. Susceptibility tests . Like an animal or a plant, the life of bacteria involves a daily routine of thousands of chemical reactions, many devoted to the breakdown (catabolism) of substrates to extract energy or building materials. Materials Required : ... one can also test for indole production by adding 5 drops of Kovacs’ reagent to the SIM cultures and looking for the development of a red color at the top. The physical test results of the API Staph test, when placed into the apiweb resource, come back with a 99.1% identity as a Micrococcus species, with an oxidase positive and yellow colony morphology leaning the test results towards Micrococcus luteus. Micrococcus luteus (left) Alcaligenes faecalis (middle) • Reduction of nitrate to nitrite to be ... •Results: + clotting in the bottom of the broth • Reagents: Plasma ... • To test for the ability of bacterium to migrate in solid agar deep. Laboratory diagnosis Microdase (Modified Oxidase) Test 1. The oxidase test results suggest that the microbe does not contain oxidase, despite what the metagenome binning test showed. Expected Results: Motility is observed in case of Proteus vulgaris, while Micrococcus luteus is non motile . Results and discussion. staphylococcus flavocyaneus, (schroeter, 1872) cohn 1872 hutner 2 atcc 8673; hutner 2 micrococcus luteus isolated at haskins laboratories new york The National Collection of Type Cultures comprises over 5000 bacterial cultures, over 100 mycoplasmas and more than 500 plasmids, host strains, bacteriophages and transposons. Micrococcus species are strictly aerobic Gram positive cocci arranged in tetrads or irregular clusters, and cells range from 0.5 to 3 µm in diameter. They are also catalase positive and often weakly oxidase positive ( modified oxidase test … M. luteus was cultured in three blood cultures at three separate times during a period of 7 weeks. Micrococcus luteus= yellow pigment 2. The modified benzidine test was compared with a modified oxidase test for differentiation within the Micrococcaceae and shown to be positive for all members of the genus Micrococcus and for strains of S. sciuri. They are seldom motile and are non-sporing. M. luteus is known as an opportunistic pathogen for nosocomial infections [], and has been proved to be able to cause bacteremia, pneumonia, endocarditis, lymphoma, septic arthritis and many other diseases [3,4,5]. It is urease and catalase positive. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. 7-3 Ex. Metabolism, Physiology, and Growth Characteristics of Cocci Metabolism. 2. Staphylococcus species 2. Conclusion: In conclusion, the bacteria data supported that the bacteria are Micrococcus luteus.The data presented in the lab matched with the characteristics and test results required for Micrococcus luteus. Morphology of Micrococci. Hanging Drop Slide . Wikipedia also says that Micrococcus luteus is an obligate aerobe, backing up what my results show (2019). Despite occurrences where the appearance slightly varied (in color or rotundness), the data was spot on for the bacteria identified. Raising the pyruvate concentration to 5% pyruvate enabled neutralization of up to 300 ppm VHP, permitting subsequent growth of Micrococcus luteus on agar media. Micrococcus roseus= pink pigment ... –Catalase test –Coagulase test –DNAse test –Oxidase test 4. The 1% pyruvate retained its neutralizing activity for Micrococcus luteus at up to 100 ppm VHP.
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